Organisers of the 15th annual Salvation Army Charity and Toy ride are keen to let people know anyone can join the ride.
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Organised by the Horsham chapter of the Harley Owners Group International, money and gifts collected are donated to the Salvation Army.
Chapter director Shane Rees said even though his chapter organised the ride, everyone was welcome.
"The idea is we want to have as many people as possible, and it doesn't matter what type of motorcycle you have," he said.
Mr Rees said the muster point for Thursday's ride was in Natimuk at 9.30am.
He said the ride was from Natimuk to Sawyer Park in Horsham, via the longer, scenic route.
"It is only a 25 minute trip going through the Three Bridges Road area," he said.
Motorcycle enthusiast John Aisbett said it would be the first time he had participated in the ride.
"I went on a longer ride recently and met a few people who had gone on the charity ride before," he said.
"A few of the riders were talking about the toy run and just trying to get as many people involved as possible."
Mr Aisbett said the enthusiasm of the other riders convinced him to sign up for the ride.
"It sounded like a great cause to be involved with," he said.
"I had known about it but didn't realise it was for anybody who wanted to tag along."
Mr Aisbett said often people had to belong to a club to go on motorcycle rides.
"It doesn't matter whether you have a Harley like me, a trail bike or a little scooter, everyone is welcome on the ride," he said.
Mr Aisbett said his bike was a 2004 Harley Davidson Fat Boy.
"I don't do a whole lot of riding but when I do I enjoy it," he said.
Mr Rees said there was a prize for the bike dressed up with the best Christmas theme.
He said people could still be involved, even if they did not have a motorcycle.
"If people want they can come to either Natimuk between 9.30am and 10.30am or Sawyer Park and ask the riders to pass on money or gifts," he said.
"It has happened before and we would love to see it happen again."