The Bureau of Meteorology is predicting the mercury to drop below zero for each of the next four days in Horsham.
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After tempartures dropped to negative 1.2 early on Wednesday morning, duty forecaster Keris Arndt said the city's residents could expect similar conditions until Tuesday.
"Thursday will probably be the warmest night given there will be some cloud cover, but as we move into the weekend every day has the potential to drop to one or two degrees below freezing, with frost and fog in the morning," he said.
Warracknabeal, Nhill and Edenhope are also expected to experience sub-zero conditions on Friday morning.
Mr Arndt said forecasts suggested there would not be snow in the Grampians in this cold snap.
"It's cold enough, but there is no rain forecast for the area," he said. "At this stage the next significant rainfall is predicted for Friday week."
"Warnings for sheep graziers usually go out after a sharp change in conditions. That's not happening in current situation given it's expected to be a prolonged period of frosty conditions, but if anyone has susceptible crops or livestock it is something to be aware off every morning until Monday."
VicRoads suggests motorists use fog lights instead of setting headlights on high beam in foggy conditions, or that they use their hazard lights with headlights on low beam.
The body also suggests accelerating smoothly and braking gently.
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