The Civil Aviation Safety Authority says Nhill and Warracknabeal residents may notice "an uncommon flying pattern" amongst aircraft taking off from the two airfields from Tuesday.
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The organisation is carrying out flight path safety checks at nine Victorian regional airports, including the two Wimmera locations, between February 11 and 16.
In a statement, the CASA said: "A twin-engine Cessna Conquest aircraft will be used to carry out the safety checks within an area up to 35 kilometres from the aerodromes.
"Low-level flying is an essential part of the safety exercise, with the aircraft down as low as several hundred feet at times.
"Local residents may notice an uncommon flying pattern, but this is to make sure obstacles are accurately marked on charts and no new obstacles exist. Obstacles can be towers, trees, masts or buildings that can be a danger to aircraft.
"The Civil Aviation Safety Authority requires aerodrome flight path safety checks to be carried out every three years to maintain a high level of air safety at all aerodromes."