The first case of coronavirus has been recorded in the Horsham Rural City Council municipality.
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An update from the Department of Health and Human Services on Saturday afternoon confirmed that one case of COVID-19 been recorded in the region.
A specific location was not provided.
A third case has also been recorded in the Northern Grampians Shire.
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The report stated that the total number of coronavirus cases in Victoria has risen by 111 in one day to 685.
Victoria has recorded three deaths related to COVID-19.
The Wimmera recorded its first COVID-19 case on March 22, in the Yarriambiack Shire.
The Northern Grampians recorded its first case on March 25, and its second on March 27.
Victoria's chief health officer Professor Brett Sutton urged the public to take the threat of coronavirus seriously.
"Everyone who's unwell must isolate themselves and everyone who's been told they're in quarantine either as a returned traveller or close contact must do so," Professor Sutton said.
"Social distancing will save lives.
"Everyone needs to comply with restrictions in place to keep yourself, your loved ones and the whole community safe.
"We urge everyone to stay 1.5 metres away from everyone else, wash your hands often with soap and water and cough or sneeze into a tissue or your elbow."
A spokeswoman for the Wimmera Base Hospital declined to comment on the case.
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