Timothy Walsh, 37, is accused of driving a B-Double that left the road and lost its trailer on the Western Highway in the early hours of July 11, 2019.

It's alleged a bus carrying more than 40 passengers collided into the detached trailer.

IN OTHER NEWS: Wimmera police seek more female recruits after recent success

The bus driver, Emil Pich, 60, died at the scene, and more than 40 people were treated at the Wimmera Base Hospital after the incident.

Mr Walsh will stand trial on charges of culpable driving, dangerous driving, causing death, dangerous driving causing serious injury and drug-related driving charges.

The South Australian resident pleaded not guilty to the offences in the Horsham Magistrates' Court on Friday.

MAKING NEWS ACROSS THE WIMMERA:

The Major Collision Investigation Unit laid charges against Mr Walsh in July this year.

Mr Walsh and his passenger were not injured in the incident.

Mr Walsh will appear before the Horsham County Court later this year.

If you are seeing this message you are a loyal digital subscriber to the Wimmera Mail-Times, as we made this story available only to subscribers. Thank you very much for your support and allowing us to continue telling the Wimmera's story. We appreciate your support of local journalism.

Did you know you can receive updates straight to your inbox? To make sure you're up-to-date with news from across the region, sign up below.